Abstract: Acrylamide (AA) and glycidamide (GA), a metabolite of AA in body are known as the possible carcinogen and cytotoxic materials on human health. In the present study, it was investigated that whether cyanidin 3-glucoside (Cy-3-glu), a kind of food-borne ingredient, can decrease the GA-reduced cytotoxicity of human breast cancer MDA-MB-231 cells. Colorimetric determination, enzyme activity detection and immunoblot analysis were performed. Results show that Cy-3-glu can significantly reduces GA-induced cytotoxicity and intracellar reactive oxygen species (ROS) overproduction. Moreover, the pretreatment with Cy-3-glu suppresses glutathione (GSH) depletion, glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities, and enhances GPx1 and GSTP1 protein expression by GA. Cy-3-glu could remarkably inhibit the cell damage induced by GA in MDA-MB-231 cells.