Abstract: The accurate detection of the boundary 1ayer separation point in real time has a great benefit to improve micro-aircraft's climb, maneuvering and pneumatic abilities. By analyzing the principle and framework of real time data collecting and processing system, a real time signal collecting and processing system, which could be applied to detect the boundary 1ayer separation point of micro-aircrafts, has been proposed and developed. The system mainly consists of analog low-pass filter, analog to digital converter (ADC), FPGA controlling circuits and USB interface circuits. The testing results show that the performance of developed system is good, which is well suitable for detecting and estimating the boundary 1ayer separation point in real time.