对联苯二酚桥联环糊精键合硅胶固定相的合成与色谱性能测试

Preparation and Characterization of 4,4-Dihydroxydiphenyl-Joined β-Cyclodextrin Bonded Silica Stationary Phase for High Performance Liquid Chromatography

摘要: 合成对联苯二酚桥联环糊精键合硅胶固定相;利用傅里叶红外光谱、X射线光电子能谱、莫氏(Molisch)颜色实验进行了表征;以邻苯二甲酸二甲酯、甲苯和菲为测试溶质,评价该键合固定相的色谱性能,并考察了该固定相对一些位置异构体的色谱分离效果;讨论了可能的分离机理.

Abstract: A 4,4-dihydroxydiphenyl-bridged β-cyclodextrin bonded stationary phase(β-CD-D2-CSP) was prepared. The resulting bonded silica has been characterized by various methods. The chromatographic performances of β-CD-D2-CSP used for isomers' separation were investigated, and their mechanism of retention was discussed.