Abstract: To understand the working characteristics of Li-ion battery and evaluate its performance of application on electric vehicles, charging and discharging experiments of Li-ion battery were carried out according to the specific requirements of electric vehicles. Based on the experimental results, the characteristic curves of Li-ion battery’s working voltage, voltage dropping rate and rise of temperature during the charging and discharging processes were studied, and the charging and discharging characteristic of Li-ion battery was analyzed in detail. Especially the influence of Li-ion battery cell’s consistency on the whole battery pack’s performance and cycle life were evaluated and analyzed. As a conclusion, application of Li-ion battery in electric vehicles seems to be very promising, but the battery’s consistency still needs to be improved.