Development and Validation of a HPLC Method for Determination of Flavone Glycoside-Camellianin B in Cephalotaxus Sinensis

A simple, rapid and sensitive method for the quantification of camellianin B in Cephalotaxus sinensis (C. sinensis) a natural plant with anti-hyperglycemic effect, was developed and validated by reversed phase liquid chromatography. Chromatographic separation was achieved on a C₁₈ column and an isocratic elution was carried out at a flow-rate of 1.0 mL·min^-1 with the acetonitrile-water containing 0.1% formic acid (19:81, v/v). The detection wavelength was set at 330 nm. The calibration curve was linear over the concentration range of 0.25-50 μg·mL^-1 with correlation coefficients larger than 0.999 5. The limit of detection and limit of quantification were 0.09 μg·mL^-1and 0.25 μg·mL^-1, respectively. The precisions and accuracy for all samples were acceptable. The validated method has been successfully applied for the quantification of camellianin B in C. sinensis harvested in different months and may also be used as the quality evaluation of this herb medicine.